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Western blotting confirmed that the level of Aβ produced by the L30F/T164A mutant was roughly two times the quantity produced by the WT (determine 6b). We used ELISA to quantify Aβforty and also aβ42 generation with the L30F/T164A mutant; we observed which the L30F/T164A mutation resulted in approximately twice just get more info as much Aβ40 plus aβ42, when compared While using the quantities produced by the WT (determine 6c). The ratio of very long Aβforty two having aβforty was similar between the WT and mutant (determine 6d).
Setelah ditelusuri lebih lanjut ditemukan bahwa uang sebanyak Rp 23,5 Miliar akhirnya mengalir kepada rekening pribadi Liddell. Kasusnya membutuhkan waktu lama untuk diproses hingga hukuman yang telah ditetapkan dijatuhkan kepada Liddell.
five-fold better activity than did the WT (Figure 3a). Immunoblotting verified the expression amounts of mutant Aph1 and PS1 had been similar to ranges while in the WT. These success propose that γ-secretase Using these two Aph1 mutations exhibit Improved protease exercise for application.
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